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Figure 1

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ZDB-FIG-201003-4

Publication

Quan et al., 2020 - Somatostatin 1.1 contributes to the innate exploration of zebrafish larva

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Figure 1

Generation of the sst1.1 mutant and a high-throughput assay for kinematic analysis. (a) Generation of the mutated allele icm40 of the somatostatin 1.1 gene (sst1.1^icm40) using CRISPR/Cas9-mediated genome editing. Top, genomic organization of the sst1.1 wild type gene with the coding sequence (yellow) of the signal peptide (sp, 24 amino acids) and (orange) mature somatostatin peptide (sst, 26 amino acids). Bottom, the icm40 mutated allele results from 23-bp deletion that impairs the start codon, and induces a frameshift in the sst1.1 coding sequence. (b) We developed a high throughput behavior assay to simultaneously record and at high frequency (350 Hz) 32–88 freely-swimming zebrafish larvae in individual circular swim arenas of 15-mm diameter. Behavioral recordings were tracked using the ZebraZoom algorithm⁴⁹, which detects the head direction (white line), the body position (red dots) and the tail end position (blue dot). See Supplementary Video S1. (c) Illustration of the pipeline for image processing in order to achieve rapid high-throughput analysis of kinematics between mutants and their control siblings. See “Methods”.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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