Fig. 1
- ID
- ZDB-FIG-200917-6
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- Zhou et al., 2020 - Conservation as well as divergence in Mcidas function underlies the differentiation of multiciliated cells in vertebrates
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Overexpression of mouse Mci in zebrafish embryos triggers ectopic foxj1 expression and formation of ectopic MCCs. (A, B) foxj1a (A) and foxj1b (B) expression in 24 hpf zebrafish embryos, as detected by the anti-GFP antibody staining for GFP, were elevated and expanded in mouse Mci (mMci) mRNA-injected foxj1a::GFP (A) and foxj1b::GFP (B) transgenic reporter fish lines, compared to uninjected controls. Ectopic foxj1a expression was detected in the head (arrow) of the majority (90%, n = 20) of Mci-injected foxj1a::GFP fish (A, with magnified dorsal views of the heads in the panels on the right). In the majority (65%, n = 14) of Mci-injected foxj1b::GFP embryos (B), ectopic foxj1b expression was detected in various tissues, including the otic vesicle (arrow, with magnified lateral views of the otic vesicles in the panels on the right). Scale bars = 100 μm. (C, D) Ectopic multiciliation caused by mMci mRNA overexpression in the zebrafish embryo. (C) Density of multicilia bundles in the pronephric ducts increased in mMci mRNA injected embryos compared to uninjected controls (62.5%, n = 16) from two independent experiments. (D) Ectopic multiciliation in the eye in mMci mRNA injected embryos compared to uninjected controls (50%, n = 16 from two independent experiments). Scale bars = 5 μm. For this and all other figures Ace-tub = anti-acetylated tubulin (labeling cilia), γ-tub = anti-γ-tubulin (labeling basal bodies), DNA = DAPI (labeling nuclei). |
Reprinted from Developmental Biology, 465(2), Zhou, F., Rayamajhi, D., Ravi, V., Narasimhan, V., Chong, Y.L., Lu, H., Venkatesh, B., Roy, S., Conservation as well as divergence in Mcidas function underlies the differentiation of multiciliated cells in vertebrates, 168-177, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.