FIGURE

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ID

ZDB-FIG-200709-32

Publication

García-Poyatos et al., 2020 - Scaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish

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Transgenic expression of <italic>scaf1</italic> recovers <styled-content toggle='no' style='fixed-case'>CIII</styled-content> and <styled-content toggle='no' style='fixed-case'>CIV</styled-content> super‐assembly and fish size of <italic>scaf1</italic><sup>−/−</sup> fish

Scheme of the transgenic construct Tg(ubi:scaf1). ubi, ubiquitin promoter. Βg‐intron, beta‐globin intron, pA, poly A, crys, crystalline promoter (used as selection marker).

Immunodetection of the indicated proteins of BNGE from whole fish mitochondria scaf1^Δ1/Δ1 (−/−) expressing the transgenic construct Tg(ubi:scaf1) in heterozygosis, their scaf1^Δ1/Δ1 siblings with no transgenic expression, and scaf1^+/+ zebrafish (representative BNGE of n = 5). Asterisks mark bands’ absence in scaf1^Δ1/Δ1 (−/−) recovered by the transgenic expression of scaf1.

C, D

Representative images from scaf1^+/+, scaf1^Δ1/Δ1 (scaf1^−/−), and scaf1^−/−, Tg/− (C) female and (D) male zebrafish (3 mpf).

E, F

Size of scaf1^+/+, scaf1^Δ1/Δ1 (scaf1^−/−), and scaf1^{−/−, Tg/−} fish (E) length of females (scaf1^+/+n = 23, scaf1^−/−n = 39 and scaf1^{−/−, Tg/−}n = 25); and (F) males (scaf1^+/+n = 15, scaf1^−/−n = 31 and scaf1^{−/−, Tg/−}n = 44).

Data information: One‐way ANOVA. ns P > 0.05, **P < 0.01, ****P < 0.0001. Data are represented as mean ± SD.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT cox7a2l^brn1/brn1 cox7a2l^brn2/brn2
Condition:	chemical treatment by environment: carbonyl cyanide p-trifluoromethoxyphenylhydrazone
Observed In:	cardiac muscle cell mitochondrial intermembrane space cardiac muscle cell mitochondrion muscle cell mitochondrial chromosome whole organism oxygen transport
Stage Range:	Long-pec to Adult

Phenotype Detail

Acknowledgments

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