FIGURE

Fig 3

ID

ZDB-FIG-200629-3

Publication

Gangras et al., 2020 - Zebrafish rbm8a and magoh mutants reveal EJC developmental functions and new 3'UTR intron-containing NMD targets

Other Figures

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Fig 3

EJC mutant embryos are paralyzed, have disorganized muscles and stunted motor axons.

A. Boxplots showing the number of spontaneous contractions per minute measured for the EJC mutant embryos and WT siblings at 24 hpf as indicated on the x-axis. Welch’s t-test p-values are indicated at the top. B-D. Immunofluorescence images showing Myh1 expression in somites 10–14 of WT sibling (B) rbm8a mutant (C) and magoh (D) mutant embryos. Antibody used was anti-A4.1025 (see methods) (N = 10 embryos/genotype). E-G. Merged confocal images of somites 12–16 in WT siblings (E) rbm8a (F) and magoh (G) mutant embryos showing immunofluorescence detection of motor neurons (anti-SV2; red) and acetylcholine receptors (α-Bungarotoxin; green). Neuro-muscular junctions in the merged image appear yellow. White arrowheads point to the end of the motor neuron. Scale bar in G is 100 nm. H. Boxplots showing the quantification of motor axon length in somites 12–15 of wild-type sibling, rbm8a mutant, and magoh mutant embryos (N = 4 embryos/genotype and 4 neurons/embryo). Welch’s t-test p-values are at the top.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	magoh^oz37/oz37 rbm8a^oz36/oz36
Observed In:	motor neuron axon muscle cell muscle contraction myotome whole organism whole organism neuromuscular junction
Stage Range:	14-19 somites to Prim-5

Phenotype Detail

Acknowledgments

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