Loss of adnp leads to the defective neural development in zebrafish embryos.
a CRISPR/Cas9 gRNA design and the genotyping result of the adnpa mutant allele. b gRNA design and the genotyping result of adnpb mutant allele; c representative morphology of 2 dpf control and adnpa adnpa double mutants. Black arrow showing the obvious cell death (black area) in head region of mutant embryos. d 3D Z-stack image showing the reduced expression of HuC/D in brain of 36 hpf control and double mutant zebrafish embryos. Arrows show the distance between the convex tip of the eye cups. e GFP fluorescence signal showing the reduced elavl3-GFP levels in adnpa adnpb deficient embryos. f Whole-mount in situ hybridization (WISH) images for the indicated neural markers for 2 dpf control and double mutant embryos. g WISH images for the indicated neural markers for 13 hpf control and double mutant embryos. h WISH images for the indicated neural markers for 24 hpf control and double mutant embryos. Black arrows pointing to where expression levels were reduced. All experiments were repeated at least two times, and similar results were obtained. Scale bars: 200 μm in (c), 100 μm in (d–h).