Figure 5—figure supplement 1.

Atoh1a+ cells became SCs and MCs post severe injury.

(A) The Neo-treated atoh1a:TdTomato;brn3c:GFP larvae was imaged for time lapse. Results showed that one Tomato+ cell turned into two HCs with membrane-bound GFP post neo (blue dots). (B) The LY+neo-treated atoh1a:TdTomato;sox2:GFP larvae was imaged for time lapse. The blue dots indicate that one Tomato+ cell divided and turned into two sox2:GFP-negative HCs post LY+neo. The white dots indicate that two Tomato+ cells divided and became four sox2:GFP-positive cells post LY+neo. (C and D) The atoh1a:TdTomato;ET4;ET20 larvae were collected at 48 hr post neo or LY+neo. Tomato expressing HCs, SCs and MCs were separately divided by total numbers of each to calculate Tomato+ ratios. The results showed that significantly more Tomato+ SCs were detected post LY+neo compared with neo. In addition, many Tomato+ MCs were detected post LY+neo, but none was detected in 5dpf and neo.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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