Fig 2

(A-H) Confocal images of primary spermatocytes at different prophase stages of meiosis I as indicated by anti-Sycp3 antibody staining (red). Arrowheads indicate spermatozoa nuclei from wild-type testis stained with DAPI in blue (A-E). (I) Bar graph showing the statistical results of the percentage of cells in different meiotic stages. The numbers of spermatocytes investigated are shown on top of each bar. (J-K) Staining of γH2AX (red) in the testes of wild-type and e2f5 mutant. Arrows point to the primary spermatocytes at leptotene and zygotene stages. (L-M) Confocal images showing apoptotic cells stained by TUNEL assay in red. (N) Bar graph with individual data points showing the number of TUNEL positive cells in wild-type and e2f5 mutant testes. (O) Phenotypes of e2f5;tp53 double mutants. (P) Bar graph showing the percentage of abnormal embryos produced from e2f5;tp53 double mutant females crossed with wild-type males. n = 441 from two double mutant females. (Q-T) Histological analysis of ovaries from wild-type and e2f5;tp53 double mutants. Inserted images are magnified views. Arrow in Q indicates peripheral localization of nucleoli stained by DAPI in stage II oocytes of wild-type ovary. The asterisk in S indicates cortical alveoli at later stage oocytes, which were visible under the confocal microscope due to autofluorescence. In panels J-M, Q-T, nuclei were counterstained with DAPI in blue. Scale bars: 10 μm in panel A-H, J-M and 50 μm in panel Q-T.