FIGURE

Fig 3

ID

ZDB-FIG-200325-59

Publication

Takemoto et al., 2020 - Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes

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Fig 3

SC formation is impaired in <italic>sycp2</italic> mutant spermatocytes.

Immunostaining of SC components on wild-type (A), sycp2^-/- and sycp2^its/its (B) spermatocyte chromosomal spreads. Individual images with anti-Sycp3, anti-Sycp2, or anti-Sycp1 antibodies and a merged image are shown for each nucleus. Wild-type nuclei are staged according to the staining patterns of Sycp3, Sycp2 and Sycp1 (A-i to A-v) [26]. The white line on A-ii indicates a border with another nucleus on the top right. Nuclei from two individual sycp2^its/its males (M1 and M2) are shown (B-ii to B-v). Wild-type-like nuclei were observed only among M1 spermatocytes. All spreads were prepared and stained at the same time, and all images were processed in the same manner. Scale bars, 5 μm.

Expression Data

Genes:	sycp1 sycp2 sycp3
Antibodies:	Ab1-sycp1 Ab1-sycp2 Ab1-sycp3
Fish:	WT sycp2^{t31556/t31556} sycp2^{zf3287/zf3287}
Anatomical Term:	spermatocyte chromatin
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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