FIGURE

Figure 3

ID

ZDB-FIG-191230-1732

Publication

Simbulan-Rosenthal et al., 2019 - CRISPR-Cas9 Knockdown and Induced Expression of CD133 Reveal Essential Roles in Melanoma Invasion and Metastasis

Other Figures

All Figure Page

Back to All Figure Page

Figure 3

BAK-R cells exhibit increased invasion and metastasis in transwell invasion and zebrafish assays, compared to BAK-P (b,c left panels), while CD133 siRNA knockdown reduces CD133 and MMP2 expression (a) and inhibits invasion (b,c right panels) *** represent p < 0.001. (c) Transwell cell invasion assays were performed for 48 h. Cells that have invaded to the bottom of the transwell were stained with toluidine blue for imaging. Percentage of cell invasion was quantified and presented as a bar graph. Zebrafish were injected with the BAK-P (d) and BAK-R cells alone (e) or in combination (f), and imaged at day 1 (left panels) and day 5 (right panels). Quantification revealed that whereas parental cells showed no metastasis (g), reprogrammed CD133(+) BAK-R cells grafted alone (h), or in combination with parental BAK-P cells (i), exhibited significant metastasis to the tail (j).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cancers