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Fig. 13

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ZDB-FIG-191220-41

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Vetrivel et al., 2019 - Mutation in the mouse histone gene Hist2h3c1 leads to degeneration of the lens vesicle and severe microphthalmia

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Fig. 13

Mutated Hist2h3c1 over-expression perturbs zebrafish development and eye formation. a-c’: Injection of mutated AEY69 Hist2h3c1 mRNA into zebrafish embryos led to developmental delay (c), malformation and cyclopia (c’), while wild-type C3H mRNA was not eliciting any defect (b), compared to not injected controls (a), when analyzed at 24 hpf. d-g’‘: The analysis at 2 dpf confirmed normal and comparable phenotypes in not injected (d), control phenol-red-injected (ph.red) (e) and C3H-injected (f) embryos, while AEY69-injected embryos showed developmental delay (g), malformation and cyclopia (g’, g’‘). The isl1 marker (blue) labels retina (re), cranial ganglia (cg) and pancreas (p), while cryba2b (red) identifies the lens (le) region. a-c’ and g’’ panels display lateral views with anterior to the left; d-g’ panels display dorsal views with anterior to the top. Displayed phenotypes are representative of n = 60 embryos per condition. Scale bar in A is 200 μm and applies to all images.

Expression Data

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Antibody Labeling

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Reprinted from Experimental Eye Research, 188, Vetrivel, S., Tiso, N., Kügler, A., Irmler, M., Horsch, M., Beckers, J., Hladik, D., Giesert, F., Gailus-Durner, V., Fuchs, H., Sabrautzki, S., German Mouse Clinic, Helmholtz Zentrum München, German Research Center for Environmental Health GmbH, Neuherberg, Germany, Adler, T., Treise, I., Busch, D.H., Aguilar-Pimentel, A., Ollert, M., Götz, A., Amarie, O.V., Stoeger, T., Schulz, H., Becker, L., Klopstock, T., Schrewe, A., Spielmann, N., Bekeredjian, R., Garrett, L., Hölter, S.M., Zimprich, A., Wurst, W., Mayer-Kuckuk, P., Hans, W., Rozman, J., Klingenspor, M., Neff, F., da Silva-Buttkus, P., Calzada-Wack, J., Rácz, I., Zimmer, A., Rathkolb, B., Wolf, E., Prehn, C., Adamski, J., Östereicher, M., Miller, G., Steinkamp, R., Lengger, C., Maier, H., Stoeger, C., Leuchtenberger, S., Gailus-Durner, V., Fuchs, H., Hrabě de Angelis, M., Hrabě de Angelis, M., Graw, J., Mutation in the mouse histone gene Hist2h3c1 leads to degeneration of the lens vesicle and severe microphthalmia, 107632, Copyright (2019) with permission from Elsevier. Full text @ Exp. Eye. Res.