Fig. 5

Effect of the endocannabinoid receptor antagonists, AM251 and AM630 on morphological development in 5 dpf zebrafish larva. (A) Incidence of pericardial edema and yolk sac edema in 5 dpf larvae when treated with AM251 and AM630 in the first and second 24 h of development. Scale bars: 0.7 mm. (B,D) Rates of pericardial and yolk sac edema and tail and body malformation in 5 dpf larvae treated with AM251 and AM630 in the first 24 h of development (n=62, 56, 51 and 58 for vehicle control, 0.5 µmol l⁻¹ AM251, 5 µmol l⁻¹ AM630 and 0.5 µmol l⁻¹ AM251+5 µmol l⁻¹AM630, respectively). (C,E) Rates of pericardial and yolk sac edema and tail and body malformation in 5 dpf larvae treated with AM251 and AM630 in the second 24 h of development (24–48 hpf) (n=74, 56, 62 and 64 for vehicle control, 0.5 µmol l⁻¹ AM251, 5 µmol l⁻¹ AM630 and 0.5 µmol l⁻¹ AM251+5 µmol l⁻¹ AM630, respectively). Data are presented as means±s.e.m. ^aSignificantly different from vehicle control, P<0.05; ^bsignificantly different from 0.5 µmol l⁻¹AM251, P<0.05; ^csignificantly different from 5 µmol l⁻¹ AM630, P<0.05 (one-way ANOVA followed by Tukey’s post hoc multiple comparisons test).