FIGURE

Fig. 6

ID
ZDB-FIG-191031-9
Publication
Prummel et al., 2019 - A conserved regulatory program initiates lateral plate mesoderm emergence across chordates
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Fig. 6

The zebrafish + 2.0 drl enhancer reads out a LPM program across vertebrates.  a c Representative HH9 ex-ovo-cultured chicken embryo electroporated at HH3+/4 with + 2.0drl:EGFP (green,  a c) and ubiquitous  pCAGGS:mCherry control (magenta,  a c), showing specific + 2.0drl reporter expression in the electroporated LPM ( n = 26/32). The dashed line depicts the outline of the chicken embryo, anterior (A) to the left, posterior (P) to the right; boxed region ( a) depicts magnified area shown for single and merged channels ( b c).  d k Expression of +2.0 drl:EGFP upon transient injections in axolotl at the indicated embryonic stages. Note EGFP expression in the lateral portion of the embryo, future gut region, and pharyngeal arches (arrowheads in  e f).  h i EGFP expression in mesenchymal cells of the developing axolotl limb bud, indicative of LPM origin ( n = 14/56).  j k EGFP fluorescence in axolotl st 43 larvae in the gut lining (asterisk,  n = 26/56) and blood vessels (arrowheads,  n = 2/56). Expression is also found occasionally in a small fraction of muscle fibers (arrows).  l o Transient transgenic lamprey embryos ( Petromyzon marinus) with + 2.0drl:EGFP expression in the anterior mesendoderm (arrowheads) and overlying the yolk at neurula stages (st 19–21) ( l m), and in the developing pharynx (arrow) during head protrusion (st 23–24) ( n); views anterior ( l), ventral ( m), lateral ( n), head (h) and yolk (y) ( n = 145/231).  o Schematic depiction of st 23 lamprey embryo to outline key features. Scale bar ( a c) 250 μm


Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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