FIGURE

Fig. 6

ID
ZDB-FIG-191003-9
Publication
Vázquez-Marín et al., 2019 - Yap1b, a divergent Yap/Taz family member, cooperates with yap1 in survival and morphogenesis via common transcriptional targets
Other Figures
All Figure Page
Back to All Figure Page
Fig. 6

yap1 and yap1b cooperate in regulating cell survival and tissue morphogenesis. Bright-field images of the anterior half of yap1+/+;yap1b+/+ (A), yap1−/−;yap1b+/+ (B), yap1−/−;yap1b+/− (C) and yap1−/−;yap1b−/− (D) stage 21 embryos from a double heterozygous cross showing negative epistatic interaction between yap1 and yap1byap1−/− enhanced malformations in yap1−/−;yap1b−/− double mutants (D). Caspase 3 staining reveals apoptotic cells in stage 20 yap1+/+;yap1b+/+ (E,I), yap1−/−;yap1b+/+ (F,J), yap1−/−;yap1b+/− (G,K) and yap1−/−;yap1b−/−embryos (H,L). Whole embryos are shown under the fluorescent stereo microscope (E-H). DAPI counterstained confocal images show individual apoptotic cells (I-L). DAPI counterstained confocal images of pH3-positive mitotic cells in stage 20 yap1+/+;yap1b+/+ (M), yap1−/−;yap1b+/+(N), yap1−/−;yap1b+/− (O) and yap1−/−;yap1b−/− embryos (P). Quantification of caspase 3-positive cells per area shows a significantly increased apoptosis with fewer functional copies available of the Yap paralogs (Q). Quantification of pH3-positive cells per area shows not significant differences in mutants for the Yap paralogs (R). Boxes represent the quartiles; the whiskers indicate the the maximum and minimum values. One-way ANOVA analysis followed by Fisher's LSD test was used to evaluate statistical significance. ov, optic vesicle. ****P<0.0001; ***P<0.001; **P<0.01; *P<0.05.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development