FIGURE

Fig. 1

ID

ZDB-FIG-190815-31

Publication

Amin et al., 2015 - Hoxa2 selectively enhances Meis binding to change a branchial arch ground state

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Hoxa2 Activates Meis1 and Meis2 in the IIBA

(A) ChIP-seq-binding profile of Hoxa2 at Meis1 and Meis2 genes in E11.5 IIBA. Input tracks represent control genomic DNA. Arrows highlight the binding regions tested by ChIP qPCR in (B).

(B) Hoxa2 binding to Meis1 and Meis2 by ChIP qPCR. Enrichment of each region following immunoprecipitation with Hoxa2 and IgG negative control antibody (Neg Ab) is calculated as percentage input; 1 and 2 indicate the corresponding peaks in (A). Pou6f2 is a positive control and Itih4 is a negative control (unbound region). Values represent the average of duplicate samples, and error bars indicate the SEM.

(C–F) Whole-mount ISH on E11.5 wild-type (C and E) and Hoxa2 mutant (D and F) embryos, using Meis1 and Meis2 probes. Arrows indicate the proximal domain of expression in the IIBA. See also Figure S1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 32, Amin, S., Donaldson, I.J., Zannino, D.A., Hensman, J., Rattray, M., Losa, M., Spitz, F., Ladam, F., Sagerström, C., Bobola, N., Hoxa2 selectively enhances Meis binding to change a branchial arch ground state, 265-77, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell