Figure 1—figure supplement 3.

Comparison of image quality between monochromatic and polychromatic X-rays for synchrotron micro-CT.

A juvenile (33 dpf) zebrafish was scanned using pink-beam (A) and monochromatic (B) sources. Insets show the ability for pink-beam (C) and monochromatic beam (D) to resolve the fine detail found in zebrafish photoreceptor layer. Signal-to-noise ratio (SNR) in high (eye lens) and low attenuation (brain) tissues was compared for both images (E). The monochromatic beam image has a higher SNR than the pink-beam image in both cases. While the noise in the pink beam image could be reduced by increasing acquisition times, the contrast in the monochromatic image is inherently better. A line profile (F) through the photoreceptor layer shows that the monochromatic beam is superior for discerning edges, which can be attributed to the phase contrast optimization done in Figure 1—figure supplement 1. Under these pink-beam imaging conditions, phase-contrast enhancement, which is energy-dependent, gets averaged out.
Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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