FIGURE

Fig. S3

ID

ZDB-FIG-190708-45

Publication

Breuer et al., 2019 - QDPR homologues in Danio rerio regulate melanin synthesis, early gliogenesis, and glutamine homeostasis

Other Figures

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Fig. S3

Characterization of Qdprb1.

(A) Lateral views, anterior to the left of 72 hpf embryos. p53 knockdown does not rescue the microcephaly phenotype of Qdprb1 hypomorphic embryos. (B) RT-PCR confirms the predicted inclusion of intron 3 upon injection of the splice blocking MO resulting in a strong reduction of correctly spliced mRNA (RT-qPCR, C). (D). Qdprb1 morphant phenotypes using low concentrations of each MO and a combination of both showing a synergy effect between both. (E) Lateral view, 72hpf ATG MO Qdprb1 injected embryos reproduce the phenotype of Splice MO Qdprb1 hypomorphic embryos. (F) Lateral views, anterior to the left. Co-injection of qdprb1mRNA in Qdprb1 hypomorphic embryos rescues brain development.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-qdprb.1 + MO4-tp53 AB + MO2-qdprb.1
Knockdown Reagents:	MO1-qdprb.1 MO2-qdprb.1 MO4-tp53
Observed In:	brain development head hindbrain midbrain
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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