Fig. 4

Ginger expands the Caudal Vein Plexus (CVP). (A) Ginger increases or rescues the CVP sinusoids in tg(fli1:GFP) WT siblings or plcg1^−/− mutant embryos respectively (2 dpf). Yellow arrows show enlarged CVP with big sinusoid. White arrow indicates dorsal aorta and pink arrow cardinal vein. (B) Bmp is necessary for the effect of ginger on the CVP in tg(fli1:GFP) WT siblings and plcg1^−/− mutant embryos (2 dpf). Vertical red bar represents the width of CVP. (C) Inhibition of Notch compromised the effect of ginger on the developing CVP at the sinusoidal level (1 dpf). Yellow arrows show enlarged CVP with big sinusoid. (D) runx1 and scl are ectopically expressed in the CVP/CHT area following ginger treatment. (E,F) Bmp7a/bmp2b expression (E) and notch3 expression (F), are highly promoted by ginger in the CVP/CHT of WT and plcg1^−/−mutant embryos. Red arrow indicates high expression in CVP/CHT (E,F). Pictures are the representative images of ≥30 embryos per condition. Red box indicates the CHT region. Scale bars = 250 μm.