FIGURE

Fig. 7

ID

ZDB-FIG-181206-32

Publication

Barrodia et al., 2018 - EF-hand domain containing 2 (Efhc2) is crucial for distal segmentation of pronephros in zebrafish

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Fig. 7

Role of Efhc2 and RA in MCC development. a WISH for MCC specific marker odf3 in efhc2 mismatch control (efhc2-MM) and splice-blocking morpholino (efhc2-Mo) injected embryos. The phenotype caused by Efhc2 knock-down was rescued by efhc2 mRNA. b Wild-type embryos or efhc2-Mo morphants were treated with RA. WISH showing expression of odf3 gene. efhc2-Mo morphants treated with RA show reduction of expression domain of MCC as compared to wild-type embryos treated with RA. c Wild-type embryos or efhc2-Mo morphants were treated with DEAB. WISH showing expression of odf3 gene. efhc2-Mo morphants treated with DEAB show reduction of expression domain of MCC as compared to wild-type embryos treated with DEAB. d Wild-type embryos or efhc2-Mo morphants were treated with citral. WISH showing expression of odf3 gene. efhc2-Mo morphants treated with citral show reduction of expression domain of MCC as compared to wild-type embryos treated with citral

Expression Data

Gene:	cimap1b
Fish:	WT WT + MO1-efhc2
Conditions:	chemical treatment by environment: citral chemical treatment by environment: 3-allyl-2-[2-(diethylamino)ethoxy]benzaldehyde chemical treatment by environment: retinoic acid
Knockdown Reagent:	MO1-efhc2
Anatomical Term:	pronephros multi-ciliated epithelial cell
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-efhc2
Conditions:	chemical treatment by environment: citral chemical treatment by environment: 3-allyl-2-[2-(diethylamino)ethoxy]benzaldehyde chemical treatment by environment: retinoic acid
Knockdown Reagent:	MO1-efhc2
Observed In:	pronephros multi-ciliated epithelial cell
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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