FIGURE

Fig. 1

ID

ZDB-FIG-180905-19

Publication

Genthe et al., 2017 - R-spondin-1 is required for specification of hematopoietic stem cells through Wnt16 and Vegfa signaling pathways

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Fig. 1

Rspo1 is required for HSC specification. The HSC markers runx1 (A,B,D,E) and cmyb (G,H,J,K), which identify HSCs emerging from the dorsal aorta, as well as the T lymphocyte marker rag1 (M,N,P,Q), found in thymic T cells, were examined at 24 hpf, 36 hpf and 96 hpf as indicated, by whole-mount in situ hybridization (WISH) in wild-type (WT; A,G,M), Rspo1 MO-injected (B,H,N), Rspo1 wild-type and heterozygous siblings (D,J,P), or rspo1 dtt^y135 homozygous mutants (E,K,Q). Red arrowheads indicate HSC precursors in the dorsal aorta (A,B,D,E,G,J,K) or T lymphocytes in the thymus (M,P), when present. Numbers of embryos displaying the depicted phenotype are indicated. (C,F,I,L,O,R) Histograms illustrate percentages of phenotypes. Lateral mid-trunk or ventral head views at 160× magnification.

Expression Data

Genes:	myb rag1 runx1
Fish:	WT rspo1^y135/y135 WT + MO3-rspo1
Knockdown Reagent:	MO3-rspo1
Anatomical Terms:	dorsal aorta hematopoietic stem cell thymus T cell
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rspo1^y135/y135 WT + MO3-rspo1
Knockdown Reagent:	MO3-rspo1
Observed In:	dorsal aorta hematopoietic stem cell thymus T cell
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

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