FIGURE

Fig. 5

ID

ZDB-FIG-180827-51

Publication

Zhao et al., 2018 - Deficiency in class III PI3-kinase confers postnatal lethality with IBD-like features in zebrafish

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Fig. 5

PI3P-positive vesicles are reduced in mutant IECs. a Represent images of transgenic zebrafish expressing the PI3P probe 2xFYVE-GFP under the control of ubiquitin promoter. Sh shield stage. b Cross sections of embryos at 6–8 dpf. PI3P-positive vesicles (arrow) are detectable in WT IECs, while the GFP signal in mutant IECs is weak and diffused. Scale bar, 20 µm. c PI3P-positive vesicles in somitic corss sections. Scale bar, 20 µm. d Quantification of PI3P-positive vesicles in (b, c). Data represent mean ± SD from three biological repeats (NS, non-significant; *p < 0.05; ****p < 0.0001 in one-way ANOVA with Tukey’s multiple comparison)

Expression Data

Gene:	EGFP
Fish:	zf2159Tg pik3c3^{zf2160/zf2160}; zf2159Tg pik3c3^{zf2161/zf2161}; zf2159Tg
Anatomical Terms:	blastoderm eye intestinal epithelium vesicle myotome myotome vesicle pharyngeal arch somite whole organism (all 8)
Stage Range:	Shield to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pik3c3^{zf2160/zf2160}; zf2159Tg pik3c3^{zf2161/zf2161}; zf2159Tg
Observed In:	intestinal epithelium intestinal epithelium vesicle myotome vesicle
Stage Range:	Day 6 to Days 7-13

Phenotype Detail

Acknowledgments

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