Fig. 6

Eaf1 or eaf2 regulated expression of ventral ved gene via Wnt/β-catenin1 signaling. (A, B) β-catenin1-MO (8 ng/embryo) rather than β-catenin2-MO (8 ng/embryo) could recover enhanced ved expression in eaf1 or eaf2 morphants to normal at both oblong stage (A) and 30% epiboly stage (B). Embryos were injected with STD-MO (A1, A2, B1, B2), eaf1-MO1 (A3, A4, B3, B4) or eaf2-MO1 (A9, A10, B9, B10). Embryos were injected with either a combination of eaf1-MO1 and β-catenin1-MO (A5, A6, B5, B6), eaf1-MO1 and β-catenin2-MO (A7, A8, B7, B8), eaf2-MO1 and β-catenin1-MO (A11, A12, B11, B12), or eaf2-MO1 and β-catenin2-MO (A13, A14, B13, B14). (A15, B15) The percentage of embryos exhibiting different expression level of ved was scored at the sphere stage (A15) and at 30% epiboly stage (B15). White box, normal; gray box, slightly increased; black box, strongly increased; blue box, reduced. All of the injections, including MO alone or eaf-MO combined with catenin-MO, were performed using the same batch of embryos produced by a select number of zebrafish to eliminate error caused by embryo variation. A1-A14, B1, B3, B5, B7, B9, B11, B13, lateral view, dorsal to the right; B2, B4, B6, B8, B10, B12, B14, animal view, dorsal to the right.