FIGURE

Fig. 6

ID

ZDB-FIG-180807-23

Publication

Zhou et al., 2018 - Chemokine C-C motif ligand 33 is a key regulator of teleost fish barbel development

Other Figures

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Fig. 6

Knockout of ccl33.2 and ccl33.3 in zebrafish using the CRISPR/Cas9 system. (A) Body length of injected fish and wild-type (WT) fish was not statistically different; however, the barbel length of the injected fish was significantly smaller (P = 0.019) than that of the WT fish. (B) Number of individuals with different phenotypes in the ccl33⁻ group and their associated genotypes. (C) Head region of WT and ccl33⁻ zebrafish at different days after hatching. White arrows indicate the barbels of WT zebrafish. No barbels were observed in the ccl33⁻ individual. (D) Individual with curly barbels in the ccl33⁻ group. dah, days after hatching. (E) Gross morphology of zebrafish from the WT group and the individual with double knockout of ccl33.2 and ccl33.3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-ccl33.2 + CRISPR1-ccl33.3 + CRISPR2-ccl33.2 + CRISPR2-ccl33.3 + CRISPR3-ccl33.2 + CRISPR3-ccl33.3
Knockdown Reagents:	CRISPR1-ccl33.2 CRISPR1-ccl33.3 CRISPR2-ccl33.2 CRISPR2-ccl33.3 CRISPR3-ccl33.2 CRISPR3-ccl33.3
Observed In:	barbel barbel skin development
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA