FIGURE

Fig. S6

ID
ZDB-FIG-180712-17
Publication
Letelier et al., 2018 - Evolutionary emergence of the rac3b/rfng/sgca regulatory cluster refined mechanisms for hindbrain boundaries formation.
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Fig. S6

Onset of activity and dissection of the regulatory regions Box B and Box C. (A–F) Double in situ hybridization experiments for gfp (blue) and egr2a (red) in F2 transgenic embryos to reveal the onset of activity of Box B (A–C) and Box C (D–F). Note that the onset of activity of Box B is delayed compared with Box C, suggesting that initiation and maintenance of gene expression might be under the control of different enhancer elements. egr2a expression is used as a landmark for r3 and r5. (G) Active enhancers’ epigenetic profile (H3K27ac; magenta peaks) along with the ATAC-seq signature from dissected hindbrains at 24 hpf (black and gray peaks) are shown in the chromosomal region within the sgca gene that contains both Box B and Box C elements. Box B was dissected into two overlapping fragments (Box B1–B2), and Box C into five overlapping regions (Box C1–C5), as depicted by dark-gray rectangles. (H–N) Stable transgenic lines for each of the fragments displayed in G were generated, and embryos at 24 hpf were assayed for gfp in situ hybridization. Note that Box B2 and Box C2/C3 (but not Boxes B1, C1, C4, and C5) drive gfp expression to the hindbrain boundaries. All images are hindbrain dorsal views with anterior at the top.

Expression Data
Gene:
Fish:
Anatomical Term:
Stage Range: 14-19 somites to Prim-5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA