FIGURE

Fig. 6

ID

ZDB-FIG-180608-38

Publication

Young et al., 2018 - Urban stormwater runoff negatively impacts lateral line development in larval zebrafish and salmon embryos

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Fig. 6

Developmental stormwater exposure does not alter cell proliferation within zebrafish neuromasts. Experiments were performed in Brn3c:mGFP fish, allowing for quantification of BrdU + /GFP + cells (hair cells). Fish were exposed to stormwater from 4 hpf – 4 dpf, and BrdU was pulsed at 32.5–34 hpf. (a) There was no change in the number of BrdU + hair cells in stormwater-treated embryos (black bars, two-way ANOVA, F_2,44 = 1.701, p = 0.19). There was a significant increase in BrdU-labeled hair cells in LiCl-treated zebrafish (gray bars, two-way ANOVA, F_1,44 = 13.72, p = 0.0006). Asterisks indicate significant pairwise differences between -LiCl and + LiCl groups for a given stormwater concentration. N = 7–13 zebrafish per group with exception of 50% stormwater + LiCl, where N = 4. Bars are + 1 s.d. (b) Representative confocal images showing BrdU labeling in the P2 neuromast of the trunk, with two hair cells double-labeled with GFP and BrdU (white arrows). Scale bar = 10 µm and applies to all panels.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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