Fig. 5

Blastoderm transplants between wild-type and MZnanog embryos. (A) Diagram of the blastoderm transplant. Donor embryos were injected with dextran-Alexa-488 or dextran-Alexa-546. Blastoderms were separated from yolks and combined to generate chimera embryos. (B) A chimera imaged approximately 15 min after transplant of a wild-type yolk cell and YSL with MZnanog blastoderm (B-B″″), where MZnanog tissue is labeled in green (B‴) and wild-type tissue is labeled in red (B″″). (C) A chimera at 8 hpf of a wild-type yolk cell and YSL with MZnanog blastoderm (C-C″″), where MZnanog tissue is labeled in green (C‴) and wild-type tissue is labeled in red (C″″). (D) A reciprocal chimera at 8 hpf of wild-type blastoderm with MZnanog yolk cell (D-D″″), again with MZnanog tissue labeled in green (D‴) and wild-type tissue labeled in red (D″″). (E) A chimera at 8 hpf of a wild-type yolk cell and YSL with a wild-type blastoderm from a second embryo (E-E″″), where tissue derived from each donor embryo is distinctly labeled (E‴,E″″). Composites of the fluorescent channels are shown in B″, C″, D″ and E″. Scale bar: 100 μm.