FIGURE

Fig. 4

ID

ZDB-FIG-180411-16

Publication

Winata et al., 2017 - Cytoplasmic polyadenylation-mediated translational control of maternal mRNAs directs maternal to zygotic transition

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Fig. 4

Phenotype caused by CPA inhibition. (A-C) At 3.5 and 5.3 hpf, the external yolk syncytial layer (e-YSL) could be observed in control embryos. (D-F) Embryos treated with 3′-dA beginning at the one-cell stage to 3.5 hpf still had visible e-YSL at 3.5 hpf, but this structure disappeared by 5.3 hpf, and epiboly did not proceed further (n=5). (G-J) Whole-mount in situ hybridization with the YSL marker camsap2a2 confirmed the absence of e-YSL up to 8 hpf (n=8). Other gastrulation markers, including dorsal shield (K-L; n=8) and mesendodermal cells expressing wnt8 (M,N; n=8), were also absent.

Expression Data

Genes:	camsap2a gsc wnt8a
Fish:	AB
Condition:	chemical treatment by environment: cordycepin
Anatomical Terms:	external yolk syncytial layer internal yolk syncytial layer margin shield
Stage Range:	Shield to 75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	chemical treatment by environment: cordycepin
Observed In:	epiboly involved in gastrulation with mouth forming second external yolk syncytial layer internal yolk syncytial layer margin shield
Stage Range:	High to 75%-epiboly

Phenotype Detail

Acknowledgments

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