Fig. 4 S1
- ID
- ZDB-FIG-180207-22
- Publication
- LeBert et al., 2018 - Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair.
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Pharmacological inhibition of early ROS and NFκB signaling disrupted the formation of SHG and epithelial projections. (A) Unwounded larvae rarely formed projections during development. (B) Representative bright field images of early ROS-inhibited and DMSO-control caudal fins at 24 hpw. (C) Early inhibition using WA significantly reduced projection formation at 24 hpw. (D, F, H) Polarization microscopy indicated a lack of projections in vimentin-deficient, ROS-inhibited, and WA treated larvae, respectively, at 24 hpw. (E, G, I) Z projections of SHG imaging and phalloidin labeling of the wound edge at 24 hpw in vimentin-deficient, ROS-inhibited, and WA treated larvae support the reduction of projection formation with these treatments. Data in A represents a single experiment and data in C represents data pooled from four replicates. Scale bars in A-D, F, and H represent 100 µm and E, G, and I represent 20 µm. *p<0.05. |