FIGURE

Fig. 8

ID

ZDB-FIG-170516-7

Publication

Lai et al., 2017 - Small G protein Rac GTPases regulate the maintenance of glioblastoma stem-like cells in vitro and in vivo

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Fig. 8

In vivo zebrafish xenotransplantation model of U373-MG tumorsphere cells with overexpressed Racs. Same as described in Figure 6, tumorspheroid cells derived from U373-MG harboring control GFP or Rac cDNAs were injected into the yolks of 2 dpf stage zebrafish Tg(kdr:mcherry) embryos. Confocal microscopy images were taken on the time indicated (LSM880, ZEISS). A. Inject with medium only. B. U373-vector cells. C. U373-Rac1 cells. D. U373-Rac2 cells. E. U373-Rac3 cells. Data shown were the representative from at least three independent experiments. Embryos injected number: n=10~15 for each group. GFP (green) represents glioblastoma spheroid cells, and mCherry (red) represents the vessels of the zebrafish embryos. Arrows show the angiogenesis which the new vessel branches approaching the injected tumor spheroid cells. Scale bar = 200 μm.

Expression Data

Gene:	mCherry
Fish:	ci5Tg
Condition:	cancer xenotransplantation
Anatomical Term:	vasculature
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Oncotarget