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Fig. 10

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ZDB-FIG-170512-9

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Chetty et al., 2017 - Vegf Signaling Promotes Vascular Endothelial Differentiation by Modulating etv2 Expression

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Fig. 10

All vascular endothelial cells acquire venous identity in Vegfaa MO knockdown embryos. (A-C) Vascular endothelial cell numbers counted within a selected region of two-somites in wild-type siblings and vegfaa-/-; fli1:nGFP; kdrl:mCherry embryos at 24 hpf. Only cells in the axial vasculature (not ISVs) were counted in the control embryos. Because vegfaa mutants form only a single vessel, it was not possible to assess arterial and venous cell numbers separately. There was no significant difference in cell numbers between the vegfaa mutants (n=7) and their wildtype siblings (n=8). (D-I) All vascular endothelial cells acquire venous identity in Vegfaa MO-injected stab1l:YFP; kdrl:mCherry embryos, analyzed by confocal microscopy at 48–52 hpf. Note that stab1l:YFP expression is largely restricted to the PCV and is absent from the DA in control uninjected embryos (D-F). In Vegfaa morphants, all vascular endothelial cells exhibit stab1l:YFP fluorescence (G-I). Transverse optical cross section is shown in the insets on the right; the position of cross section is marked with a dashed line (F,I).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 424(2), Chetty, S.C., Rost, M.S., Enriquez, J.R., Schumacher, J.A., Baltrunaite, K., Rossi, A., Stainier, D.Y., Sumanas, S., Vegf Signaling Promotes Vascular Endothelial Differentiation by Modulating etv2 Expression, 147-161, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.