FIGURE

Fig. 6

ID
ZDB-FIG-170505-10
Publication
Hasegawa et al., 2017 - Transient inflammatory response mediated by interleukin-1β is required for proper regeneration in zebrafish fin fold
Other Figures
All Figure Page
Back to All Figure Page
Fig. 6

il1b overexpression induces aberrant apoptosis.

(A) A schematic diagram of the construct used; in this construct, the zebrafish heat shock protein 70l promoter drives the expression of mCherry and il1b harboring a signal sequence. (B) mCherry fluorescence in Tg(hsp70l:il1b) at 4 hr after heat shock. WT, sibling WT. Scale bar, 250 μm. (C) TUNEL analysis in the amputated fin fold of Tg(hsp70l:il1b) at 2 dpa. The diagram depicts the experimental procedure. HS, heat shock. Scale bar, 100 μm. (D) Quantification of TUNEL staining in (C). (E) Fin fold regeneration in Tg(hsp70l:il1b) and sibling WT larvae. Dotted lines indicate amputation planes and fin fold outlines. Scale bar, 100 μm. (F) Quantification of the length of the regenerate (length posterior to the amputation plane; shown in (E)). In (D) and (F), data are presented as means ± SEM. Student’s t test, ***p<0.001.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage: Day 4

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife