ZFIN ID: ZDB-FIG-170328-3
Riessland et al., 2017 - Neurocalcin Delta Suppression Protects against Spinal Muscular Atrophy in Humans and across Species by Restoring Impaired Endocytosis. American journal of human genetics   100(2):297-315 Full text @ Am. J. Hum. Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Protruding-mouth

Fig. 3 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ncaldb Ab1-ncald ml2Tg control Long-pec whole organism WB
Ab1-ncald ml2Tg + MO1-ncaldb standard conditions Long-pec Not Detected whole organism WB
Ab1-ncald ml2Tg + MO1-ncaldb + MO1-smn1 standard conditions Long-pec Not Detected whole organism WB
Ab1-ncald ml2Tg + MO1-smn1 standard conditions Long-pec whole organism WB
smn1 Ab3-smn1 ml2Tg control Long-pec whole organism WB
Ab3-smn1 ml2Tg + MO1-ncaldb standard conditions Long-pec whole organism WB
Ab3-smn1 ml2Tg + MO1-ncaldb + MO1-smn1 standard conditions Long-pec whole organism WB
Ab3-smn1 ml2Tg + MO1-smn1 standard conditions Long-pec whole organism WB
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
znp-1 IHC ml2Tg control Prim-15 CaP motoneuron axon
IHC ml2Tg + MO1-ncaldb standard conditions Prim-15 CaP motoneuron axon
IHC ml2Tg + MO1-ncaldb + MO1-smn1 standard conditions Prim-15 CaP motoneuron axon
IHC ml2Tg + MO1-smn1 standard conditions Prim-15 CaP motoneuron axon
Phenotype Details
Fish Conditions Stage Phenotype
ml2Tg + MO1-ncaldb standard conditions Prim-15 CaP motoneuron axon increased branchiness, abnormal
Prim-15 CaP motoneuron collateral sprouting increased occurrence, abnormal
Long-pec fast muscle cell neuromuscular junction development process quality, normal
Long-pec fast muscle cell neuromuscular junction width, normal
Long-pec fast muscle cell synaptic cleft width, normal
Long-pec whole organism ncaldb expression absent, abnormal
Protruding-mouth fast muscle cell neuromuscular synaptic transmission process quality, normal
ml2Tg + MO1-ncaldb + MO1-smn1 standard conditions Prim-15 CaP motoneuron axon extension involved in axon guidance process quality, ameliorated
Prim-15 CaP motoneuron axon increased branchiness, abnormal
Prim-15 CaP motoneuron axon length, ameliorated
Prim-15 CaP motoneuron collateral sprouting increased occurrence, abnormal
Long-pec fast muscle cell neuromuscular junction development process quality, ameliorated
Long-pec fast muscle cell neuromuscular junction width, ameliorated
Long-pec fast muscle cell synaptic cleft width, ameliorated
Long-pec whole organism ncaldb expression absent, abnormal
Long-pec whole organism smn1 expression decreased amount, abnormal
Protruding-mouth fast muscle cell neuromuscular synaptic transmission process quality, ameliorated
ml2Tg + MO1-smn1 standard conditions Prim-15 CaP motoneuron axon decreased length, abnormal
Prim-15 CaP motoneuron axon extension involved in axon guidance decreased process quality, abnormal
Prim-15 CaP motoneuron axon increased branchiness, abnormal
Prim-15 CaP motoneuron collateral sprouting increased occurrence, abnormal
Long-pec fast muscle cell neuromuscular junction decreased width, abnormal
Long-pec fast muscle cell neuromuscular junction development decreased process quality, abnormal
Long-pec fast muscle cell synaptic cleft decreased width, abnormal
Long-pec whole organism smn1 expression decreased amount, abnormal
Protruding-mouth fast muscle cell neuromuscular synaptic transmission decreased process quality, abnormal
Full text @ Am. J. Hum. Genet.
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