FIGURE

Fig. 2

ID

ZDB-FIG-170223-35

Publication

Jindal et al., 2017 - In vivo severity ranking of Ras pathway mutations associated with developmental disorders

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Fig. 2

Ranking of MEK1 RASopathy mutations in the zebrafish heart. (A) Schematic of zebrafish orientation during heart imaging. (B) Representative images of the zebrafish heart at 19.5 hpf with the outline of the heart in yellow. Cardiac cells are labeled by membrane GFP expressed from the myl7 promoter. (Scale bar, 50 μm.) (C) Rank of selected mutations based on zebrafish heart size in embryos overexpressing MEK variants (+) compared with uninjected siblings (–, black). The average for each mutation is plotted with the SEM indicated. RASopathies, blue; RASopathies and cancer, green; cancer only, red. Here, N values are N_E203Q,– = 12, N_E203Q,+ = 9, N_G128V,– = 12, N_G128V,+ = 9, N_F53L,– = 9, and N_F53L,+ = 8. One-way analysis of variance (ANOVA) with Bonferroni correction was used for statistical analysis: *P < 0.1. The differences of the following pairs were not statistically significant: E203Q, G128V; G128V, F53L.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA