(a-d) Overview of Ninl and Ush2a localization in cryo-sections of 4 dpf zebrafish retina using anti-Ninl (red signal), anti-Ush2a (green signal) and the basal body marker anti-Centrin (cyanid signal). In all images the nuclei are counterstained with DAPI (blue signal). (a-a''''') Un-injected wildtype, (b-b''''') 10 ng/nl control MO-injected, (c-c''''') 2 ng/nl ninl MO-injected and (d-d''''') 6 ng/nl dzank1 MO-injected zebrafish retinas. (a'-d') Anti-Ush2a staining (green signal) is strongly reduced in dzank1 and ninl morphants (c'-d'), while Ush2a is clearly present in wildtype and control MO-injected larvae. (a''-d''). Specific Ninl-immunofluorescence (red signal) was largely abolished in ninl morphants and reduced in dzank1 morphants. (a'''-d''') Centrin (cyanide signal) was observed in wildtype, un-injected control and in both ninl and dzank1 morphants. (a''''-d'''') Co-localization of Ush2a and Ninl (yellow signal) was observed in wildtype and control MO-injected larvae. (a'''''-d''''') Co-localization of Ush2a and Centrin (yellow signal) was seen in all images (WT, Control Oligo, ninl and dzank1 morphants), despite strong reduction of Ush2a immunofluorescence in ninl and dzank1 morphants. Scale bars represent 15 μm, except for (a-d) in which the scale bars represent 50 μm.
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