FIGURE

Fig. 6

ID
ZDB-FIG-170208-32
Publication
Hu et al., 2016 - Liver-Enriched Gene 1, a Glycosylated Secretory Protein, Binds to FGFR and Mediates an Anti-stress Pathway to Protect Liver Development in Zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 6

N70-glycosylation is necessary for the secretion of Leg1b but not Leg1a.

(A) Western blot analysis of secretion of Leg1a, Leg1aN70A, Leg1b and Leg1bN70A. Mutating N70 to A70 does not affect the secretion of Leg1aN70A but does affect Leg1bN70A. Total protein was extracted from cell pellets or supernatant. HA-tagged RNaseI was used as a control. Western blot was repeated three times.(B) Co-immunostaining of Leg1a or Leg1aN70A with the cis- and medial-Golgi marker Giantin in HepG2 cells transfected with WT leg1a and leg1aN70A plasmids, respectively. (C) Co-immunostaining of Leg1b or Leg1bN70Awith Giantin showed that Leg1b is secreted (upper panels) but Leg1bN70A is retained in the cis-Golgi network (lower panels). (D) Co-immunostaining of Leg1b or Leg1bN70A with the ER marker PDI in HepG2 cells transfected with WT leg1b and leg1bN70A plasmids, respectively. The enlarged view of the area highlighted with dashed lines are shown on the right.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.