FIGURE

Fig. 4

ID

ZDB-FIG-161214-7

Publication

Oosterhof et al., 2017 - Identification of a conserved and acute neurodegeneration-specific microglial transcriptome in the zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Identification of gene expression changes upon neuronal cell death. (A) TUNEL and L-plastin staining showing increased neuronal cell death upon treatment with MTZ for 48 hours accompanied by microglia activation. mCherry signal represents engulfed neurons. (B) Schematic representation of cells isolated for RNA sequencing on activated microglia. (C) Volcano plot showing differentially expressed genes upon the activation of microglia (FDR <0.01; LogFC > |2|). (D) Heatmap showing Z-score values of all genes differentially expressed between activated microglia and control microglia (367 genes) (FDR <0.01, LogFC > |2|).(E) IPA canonical pathway analysis on significantly upregulated genes in microglia upon NTR-mediated ablation (FDR <0.01;LogFC > |2|). Scale bar = 40 µm in (A). For quantification in (A) cells were counted in 3 selected volumes in the olfactory bulb (4.0 × 10 ^{− 4} mm³) per fish (n = 3). Error bars represent standard deviation, *P < 0.05, ***P < 0.001 (Student's t-test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Glia