FIGURE

Fig. 5

ID

ZDB-FIG-161130-5

Publication

Grainger et al., 2016 - Wnt9a Is Required for the Aortic Amplification of Nascent Hematopoietic Stem Cells

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Fig. 5

Wnt9a Affects Endothelial gata2b+ G1-S Cell Cycle Progression through myca

(A) Endothelial cells (mCherry+) were collected by FACS from hs:dntcf:IRES-GFP; kdrl:mCherry fish at 30 hpf after heat shock at 16 hpf (n = 100 embryos per condition).

(B) Wnt inhibited (dntcf;GFP+) cells were compared to control (GFP−) cells by qPCR after being sorted by FACS.

(C and D) AB^∗ fish were injected with wnt9a MO, myca mRNA, or both; phldb4:Gal4 fish were injected with wnt9a MO, UAS;myca plasmid, and transposase mRNA, fixed at 40 hpf, analyzed by WISH for cmyb (C), and quantified in (D).

Scale bar, 100 μm. ^∗p < 0.05; ^∗∗∗p < 0.001; n.s., not significant. Error bars represent SD. See also Figure S5.

Expression Data

Genes:	ccnd1 ccnd2b cdk2 cdk4 myb myca
Fish:	AB AB + MO2-wnt9a w26Tg; y171Tg
Knockdown Reagent:	MO2-wnt9a
Anatomical Terms:	endothelial cell ventral wall of dorsal aorta hematopoietic stem cell
Stage Range:	Prim-15 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-wnt9a w26Tg; y171Tg
Knockdown Reagent:	MO2-wnt9a
Observed In:	endothelial cell ventral wall of dorsal aorta hematopoietic stem cell
Stage Range:	Prim-15 to Prim-25

Phenotype Detail

Acknowledgments

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