FIGURE

Fig. S3

ID

ZDB-FIG-161010-2

Publication

Ocaña et al., 2012 - Metastatic Colonization Requires the Repression of the Epithelial-Mesenchymal Transition Inducer Prrx1

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Fig. S3

Related to Figure 3. Efficiency and specificity of morpholino antisense oligonucleotides.

(A-B) Specificity was assessed by injecting embryos with a morpholino oligonucleotide (MO) against prrx1a or twist1b together with a plasmid containing the sequence of the prrx1a^MO (A) or twist1b^MO (B) in front of the Egfp gene. Efficiency was directly associated with the decrease in EGFP expression compared with that obtained after injection with the corresponding MO, checked at 10-12hpf by immunohistochemistry with a POD-conjugated antibody and developed with DAB (upper panels) and EGFP fluorescence emission (lower panels, fluorescence pictures taken with the same settings). Scale bar: 1 mm.

(C) The efficiency of the prrx1a splice morpholino was assessed in injected embryos by RTPCR amplification with primers from exon1 and 2, which yield a 108 bp fragment; unprocessed mRNA of 2.6 kb long cannot be amplified. The efficiency is directly related to the decrease in the amount of the 108 bp fragment.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cancer Cell, 22(6), Ocaña, O.H., Córcoles, R., Fabra, A., Moreno-Bueno, G., Acloque, H., Vega, S.,Barrallo-Gimeno, A., Cano, A., Nieto, M.A., Metastatic Colonization Requires the Repression of the Epithelial-Mesenchymal Transition Inducer Prrx1, 709-724, Copyright (2012) with permission from Elsevier. Full text @ Cancer Cell