Fig. S9

GFP expression pattern in Tg(pax1a:gfp) transgenic line. This line was identified from the founder fish that was injected with the Tol2(pax1a:gfp) construct at the one-cell stage. The expression of GFP was driven by a 2.4-kb promoter of the pax1a gene. (A, E and I) in situ hybridization indicated the expression of endogenous pax1a in the sclerotome of wild-type embryos at 24 hpf (A), 30 hpf (E) and 48 hpf (I). (B-D) GFP expression in transgenic embryos at 24 hpf. A trunk region (C) and an optical cross section (D) of the same embryo (B) were shown. n, notochord. (F-H) GFP expression in transgenic embryos at 30 hpf. A trunk region (G) and an optical cross section (H) of the same embryo (F) were shown. (J-L) GFP expression in transgenic embryos at 48 hpf. A trunk region (K) and an optical cross section (L) of the same embryo (J) were shown. (M) A GFP+ circulating blood cells (indicated by arrows) was observed by confocal microscopy in the heart at 36 hpf. (N) Flow cytometry result showing the proportion of GFP+ blood cells in embryos at 28 hpf. Blood cells were taken from the heart of 5-10 embryos at 28 hpf. The average in parenthesis was the mean of three experiments. (O) Flow cytometry results showing the proportion of GFP⁺ cells in adult blood (left) and kidney marrow (right). The showed were representative results from single fish. The average from three fish was shown in parenthesis.