Fig. 1
Clonal Association between the Three Pigment Cell Types (A) Adult zebrafish and (A′) close-up of the striped pattern on zebrafish trunk. (B-H) Types of pigment cell clones in young adult zebrafish carrying Tg(sox10:ERT2-Cre) and Tg(βactin2:loxP-STOP-loxP-DsRed-express): (B) a single melanophore (M), (C) iridophores (I), (D) xanthophores (X), (E) melanophore and iridophore (MI), (F) melanophore and xanthophore (MX), (G) iridophore and xanthophore (IX; note that this is the only IX that we obtained), and (H) melanophore, iridophore, and xanthophore (MIX) clones. Representative images in (B)-(G) (DsRed/bright field) are from clones obtained from Cre activation at 15 dpf. (I) Quantification of the clonal association between pigment cell types obtained by Cre activation at 16 hpf (n = 48 clones from 43 animals, median standard length [SL] at the time of image acquisition = 20.8 ± 1.84 mm); 5 dpf (n = 54 clones from 36 animals, median SL at the time of image acquisition = 24 ± 1.77 mm); 15 dpf (n = 112 clones from 74 animals, median SL at the time of image acquisition = 21 ± 1.77 mm); and 21 dpf (n = 38 clones from 23 animals, median SL at the time of image acquisition = 20 ± 2.07 mm). (J) Schematic representation of the segregation of pigment cell fates. N, neuron; M, melanophore; I, iridophore; X, xanthophore. Insets in (B)-(D) are blow-ups. Scale bars represent 5 mm (A) and 250 µm (B-H). See also Figures S1 and S2 and Tables S1 and S2. |
Reprinted from Developmental Cell, 38(3), Singh, A.P., Dinwiddie, A., Mahalwar, P., Schach, U., Linker, C., Irion, U., Nüsslein-Volhard, C., Pigment Cell Progenitors in Zebrafish Remain Multipotent through Metamorphosis, 316-30, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell