ZFIN ID: ZDB-FIG-160902-15
Robertson et al., 2016 - Identification of benzopyrone as a common structural feature in compounds with anti-inflammatory activity in a zebrafish phenotypic screen. Disease models & mechanisms   9(6):621-32 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Protruding-mouth

Fig. 6

Clinical cromones promote neutrophil apoptosis to drive inflammation resolution in vivo. (A) Inflammation-resolution assay in mpx:GFP larvae injected with 0.4pg/µl nedocromil or 0.5pg/µl disodium cromoglycate at 6hpi. Both compounds significantly reduce neutrophil numbers at the wound at 12hpi compared to the water control (one-way ANOVA with Dunnett′s multiple-comparison post-test; *P<0.5; n>36, performed as four independent experiments). (B-D) TUNEL assay in mpx:GFP larvae injected with water, 0.4pg/µl nedocromil or 0.5pg/µl disodium cromoglycate from 6hpi and fixed at 12hpi. Numbers of TSA-positive neutrophils and TSA/TUNEL double-positive apoptotic neutrophils at the site of injury were measured to calculate percentage neutrophil apoptosis, which was increased in nedocromil-treated larvae (one-way ANOVA with Dunnett′s multiple-comparison post-test; *P<0.05; n>54, performed as three independent experiments). (C,D) Illustrative images of water-injected (C) and nedocromil-injected (D) larvae following TSA/TUNEL staining (scale bars: 40µm). Broken lines indicate the outline of the tail-fin. White arrows in magnified view of boxed area in Diii (Div) indicate apoptotic neutrophils, identified by morphology and double TSA/TUNEL labelling. (E) Inflammation-resolution assay in the absence of macrophages. Metronidazole ablation of macrophages impairs the effect of isopimpinellin (one-way ANOVA with Bonferroni′s multiple-comparison post-test to compare selected columns; *P<0.05; ns, non-significant; n>20; performed as three independent experiments).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP i114Tg transection: caudal fin Protruding-mouth neutrophil IFL
i114Tg transection: caudal fin, chemical treatment by injection: nedocromil Protruding-mouth neutrophil IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
i114Tg transection: caudal fin Protruding-mouth regeneration epithelium neutrophil decreased amount, abnormal
i114Tg transection: caudal fin, chemical treatment by injection: nedocromil Protruding-mouth regeneration epithelium neutrophil decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.