FIGURE

Fig. 9

ID
ZDB-FIG-160818-9
Publication
George et al., 2016 - Arf6 and the 5'phosphatase of synaptojanin 1 regulate autophagy in cone photoreceptors
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Fig. 9

Constitutively active Arf6a Q67L rescues late endosome phenotype in nrca14 cones. Fixed sections of 5 days post-fertilization A: wild type (WT) and B: nrca14 Tg(TαCP : GFP-rab7) larvae transiently expressing Arf6a-tagRFPt construct in cones. WT and dominant negative Arf6a did not significantly affect the number of synaptic Rab7 puncta in WT or nrca14 cones. Constitutively active Arf6a Q67L significantly rescued the synaptic Rab7 puncta in nrca14 cones. Arf6a Q67L significantly increased the number of synaptic Rab7 puncta in WT cones. Arrowheads point to Arf6a-expressing cells (magenta in merge), arrows point to Arf6a-expresssing synaptic terminals. Scale bar = 2 µm in all images. Results were quantified by counting the number of synaptic Rab7 puncta in the Arf6a expressing cells and background corrected using the number of puncta in the non-Arf6a expressing cells in the same larvae. Graph in C shows average corrected synaptic Rab7 puncta per cell. Error bars are SEM and n ≥ 50 cells for all conditions (exact n shown on graph) (* p-value < 0.05,*** p-value < 0.001 in nrca14, #p-value < 0.05 in WT relative to cells not expressing Arf6a by one-way analysis of variance followed by Dunnett′s multiple comparison correction).

Expression Data
Gene:
Fish:
Anatomical Term:
Stage: Day 5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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