The zebrafish ventral artery diameter is increased by a NO donor and decreased by a NO synthase inhibitor. (A) In vivo imaging of the ventral artery of Tg (fli1:EGFP) zebrafish at 6 dpf. Zebrafish were incubated with or without 10 mM Sodium nitroprusside (SNP) and/or 10 mM L-NAME for 2 h and then placed on microscope slides on their backs. The ventral artery was imaged under a fluorescent microscope at 100 frames/s for 5 s. The image stack projections and time-lapse imaging traces at the level of the ventral artery (dotted lines) are shown. Scale bars, 100 µm. (B) The diameter of the ventral artery was increased and decreased by zebrafish treatment with SNP and L-NAME, respectively, relative to untreated zebrafish. The ventral artery diameter of zebrafish co-treated with SNP and L-NAME was not significantly different from that of control zebrafish. The ventral artery diameters were measured using time-lapse imaging. N = 5 for each group. *p < 0.05 vs control. (C) The length of ventral artery in zebrafish treated with SNP and/or L-NAME was not significantly different compared to that in untreated zebrafish. N = 5 for each group.
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