Fig. 2

Both white and opaque phenotypic states of C. albicans infect, disseminate, and are induced to undergo filamentation in vivo. (A) Hindbrain of wild type zebrafish in the infected with 8 C. albicans white cells (CAY4975) pre-stained with AlexaFluor-488 (see Methods) at 30 min post-injection. Magnification, 10 ×; scale bar, 50 µm. White box indicates C. albicans cells in the hindbrain and this area is shown at higher magnification in (C). (B) Hindbrain of wild type zebrafish infected with 12 C. albicans opaque cells (CAY4986) pre-stained with AlexaFluor-488 (see Methods) at 30 min post-infection. Magnification, 10 ×; scale bar, 50 µm. White box indicates C. albicans cells present in the hindbrain shown at higher magnification in (D). (C) Higher magnification of white box in (A). Magnification, 40 ×; scale bar, 20 µm. (D) Higher magnification of white box in (B). Magnification, 40 ×; scale bar, 20 µm. (E) Wild type zebrafish were infected with >200 C. albicans white cells expressing a dTomato reporter (CAY4975) and kept at 25°C. Shown are C. albicans cells that have disseminated to the tail (see arrows) at 2 days post-infection. Magnification, 10 ×. Scale bar, 200 µm. (F) Wild type zebrafish were infected with 50 opaque C. albicans cells expressing dTomato (CAY4986) and kept at 25°C. Shown are C. albicans cells that have disseminated to the tail (see arrows) at 1 day post-infection. Magnification, 10 ×; scale bar, 200 µm. (G) 10 × image of wild type zebrafish infected with 25 C. albicans white cells expressing a dTomato reporter (CAY4975) at 9 days post-infection. Arrows indicate fungal filaments penetrating out of the fish′s head. Scale bar, 200 µm. (H) 10 × image of wild type zebrafish infected with 100 C. albicans opaque cells expressing a dTomato reporter (CAY4986) and stained with AlexaFluor-488 at 5 days post-infection. Arrow indicates filamentation out of the fish′s head. Scale bar, 200 µm. (I) Confocal microscopy of zebrafish infected with C. albicans white cells (CAY4975) expressing a dTomato reporter at 1 day post-infection and exhibiting filamentation. Magnification, 20 ×. (J) Confocal microscopy of zebrafish infected with C. albicans opaque cells expressing a dTomato reporter (CAY4986) at 1 day post-infection and exhibiting filamentation. Magnification, 20 ×.