Mutations in the Tbx4 NLS cause altered expression of pelvic fin outgrowth genes. (A,B) pelvic finless zebrafish express the hindlimb initiation gene pitx1 in the developing pelvic fin mesenchyme in a similar pattern to wild-type zebrafish. (C,D) pelvic finless zebrafish express tbx4 mRNA in the developing pelvic fin mesenchyme in a similar pattern to wild-type zebrafish. (E,F) There is no detected expression of fgf10a mRNA in the developing pelvic fin regions of pelvic finless zebrafish, whilst fgf10a expression is observed in the developing pelvic fin mesenchyme of wild-type zebrafish. (G,H) Altered expression of the apical ectodermal ridge marker, sp8, is observed in pelvic finless zebrafish. In pelvic finless zebrafish the expression of sp8 mRNA is not restricted to the apical ectodermal thickening, as seen in wild-types, but is diffuse in the apical ectodermal thickening precursor cells which have failed to accumulate in the dorsoventral boundary in pelvic finless zebrafish (n=24). Scale bars: 50µm.
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