FIGURE

Fig. 2

ID

ZDB-FIG-160315-19

Publication

Govindan et al., 2016 - Cx43-Dependent Skeletal Phenotypes Are Mediated by Interactions between the Hapln1a-ECM and Sema3d during Fin Regeneration

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Fig. 2

Morpholino mediated knockdown of has1 and has2 results in reduced HA.

(A) Morpholino mediated knockdown of has1 results in reduced HA. Longitudinal section of fin rays treated with has1 control morpholino (Has1 MM) and has1 target morpholinos (Has1 MO1 and Has1 MO2). (B) Morpholino mediated knockdown of has2 results in reduced HA. Longitudinal section of fin rays treated with has2 control morpholino (Has2 MM) and has2 target morpholinos (Has2 MO1 and Has2 MO2). HA is detected by histochemical staining using biotinylated HABP and Streptavidin-Alexa 546. TO-PRO is used as the counterstain and detects DNA (blue). Compared to Has1 MM and Has2 MM treated fins, Has1 MO and Has2 MO treated fins exhibit reduced staining for HA. Arrow identifies the basal layer of epithelium; arrow head identifies the bone; m, mesenchyme; e, epithelium. Scale bar represents 20 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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