FIGURE

Fig. 2

ID

ZDB-FIG-160304-2

Publication

Kawamura et al., 2016 - Posterior-anterior gradient of zebrafish hes6 expression in the presomitic mesoderm is established by the combinatorial functions of the downstream enhancer and 3'UTR

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Fig. 2

The PT enhancer-EGFP reporter drives expression in the somites, PSM, and tailbud in transgenic embryos. (A) Reporter gene used to generate the transgenic fish line Tg(hes6:EGFP-SV40pA). The identified 1.7-kb region (PT enhancer) was inserted upstream of the promoter of hsp70l, EGFP gene, and SV40pA. (B–I) EGFP signals in Tg(hes6:EGFP-SV40pA) embryos during development. EGFP expression was initiated in the tailbud at the bud stage. The arrowhead in D indicates the posterior end of the notochord in the tailbud. During somitogenesis, EGFP signal was observed in the somites, PSM, and tailbud. (J–W) Comparisons of EGFP reporter expression with endogenous distribution of hes6 mRNA in Tg(hes6:EGFP-SV40pA) embryos. Whole-mount in situ hybridization was performed using either EGFP or hes6 antisense probes. Scale bars, 200 µm.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Developmental Biology, 409(2), Kawamura, A., Ovara, H., Ooka, Y., Kinoshita, H., Hoshikawa, M., Nakajo, K., Yokota, D., Fujino, Y., Higashijima, S.I., Takada, S., Yamasu, K., Posterior-anterior gradient of zebrafish hes6 expression in the presomitic mesoderm is established by the combinatorial functions of the downstream enhancer and 3'UTR, 543-54, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.