In situ hybridization on morpholino mediated esco2-knockdown fins shows reduced levels of cx43 expression. The MO/MM was injected in 3 dpa fins and fins were harvested at 1 dpe/4 dpa. The amputation plane is identified by black lines unless it is out of the field of view (i.e., as occurs in most of the higher magnification images). The fin in panel A appears curved because there was less growth on the esco2-knockdown side compared with the uninjected side. A: (Top) Representative image of a fin with esco2-knockdown side (esco2 MO) showing decreased staining of cx43, compared with the uninjected side (UN). (Bottom left) Higher magnification of the knockdown side (MO) of the same fin (fin rays from top image marked by *) showing reduced cx43 expression. (Bottom right) Higher magnification of the uninjected side (UN) of the same fin (fin rays from top image marked by +) showing robust cx43 expression. B: (Top) Representative image of a fin microinjected with esco2-MM in one half and the other side uninjected (UN) reveal similar levels of cx43. (Bottom left) Higher magnification of the esco2-MM injected side (MM) of the same fin (fin rays from top image marked by *) showing cx43 expression. (Bottom right) Higher magnification of the uninjected side (UN) of the same fin (fin rays from top image marked by +) showing similar cx43 expression levels. Scale bar = 100 µm.
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