Fig. 4

Split KalTA4 system restricts sox10 expression pattern to each component cell type. (A) Coinjection of elavl3:DBD and elavl3:TA4 activate reporter expression in neurons. (i) Overview of two spinal cord segments showing kaede+ neurons (arrowheads indicate examples of neuron somas). (ii) High-resolution view of spinal interneurons. (iii–iv) A pLL neuron and its axon innervating neuromasts. (B) Coinjection of sox10:DBD and sox10:TA4 activate reporter expression in neurons and glia. (i, ii) Spinal cord overviews showing kaede+ neurons (arrowheads) and OLs (arrows) forming myelin sheaths (brackets). (iii) High-resolution view of ventral OL in (i). (iv) A Schwann cell in the pLL. (v) High-resolution view of a Rohon-Beard cell in the dorsal spinal cord. (C) Coinjection of sox10:DBD and elavl3:TA4 activate reporter expression only in neurons. (i, ii) Spinal cord overviews showing kaede+ neurons (arrowheads). (iii) High-resolution view of a circumferential descending interneuron. (iv) High-resolution view of Rohon-Beard neuron and of a dorsal interneuron in (ii). All panels are lateral views at 4 dpf; all scale bars: 20 µm.