FIGURE

Fig. 1

ID

ZDB-FIG-151216-19

Publication

Cheng et al., 2015 - Transgenic expression of omega-3 PUFA synthesis genes improves zebrafish survival during Vibrio vulnificus infection

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Fig. 1

Transgenic zebrafish specifically express Fadsd6 and Elvol5a in liver. (a) Schematics of the liver-specific activator plasmid pT2-LF-Tta (containing an activator (tTA) driven by the zebrafish fabp10 promoter) and two tetracycline-responsive plasmids (pT2-TRE-fadsd6-TCFP11 and pT2-TRE-elvol5a-TCFP13). All expression cassettes are flanked by the Tol2 transposon. (b) Fluorescence microscopy images of F3 Fadsd6 (red) and Elvol5a (green) transgenic zebrafish larvae, taken at five days (5D) after hatching. (c) RT-PCR was used to detect exogenous gene expression of Fadsd6 and Elvol5a in adult transgenic and Wt zebrafish. (d) Western blots were performed to detect fusion of the V5-tag to Fadsd6 and Elvol5a in transgenic and Wt zebrafish. Mouse β-actin was used as a positive control

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biomed. Sci.