ZFIN ID: ZDB-FIG-151005-17
Mendelson et al., 2015 - Maternal or zygotic sphingosine kinase is required to regulate zebrafish cardiogenesis. Developmental dynamics : an official publication of the American Association of Anatomists   244(8):948-54 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-15

Fig. 3

The sphk2MZ mutant phenocopies the mil zygotic mutant. Shown are representative embryos at 32 hr post fertilization imaged in brightfield (column 1: lateral view, anterior to the left), (column 2: ventral view, anterior to the left), or analyzed by in situ hybridization for expression of the myl7 gene (column 3, ventral view, anterior to the left). Column 4 shows representative images of tails at this stage. Embryos were derived from crosses as indicated on the left: (A) Wildtype (WT), (B) heterozygous mil adults, (C) female and male sphk2wc1/wc1 mutants, (D) female sphk2 homozygous mutant and male sphk2 heterozygous mutant and (E) female sphk2 heterozygous mutant and male sphk2 homozygous mutant. (F) Female sphk2 homozygous mutant crossed to a wildtype male. Note that only the homozygous mil mutant embryos (25% of the embryos from the heterozygote parental cross) display pericardial edema (columns 1, 2), cardia bifida (column 3), and tail blisters (column 4), as do 100% (200/200) of the sphk2MZ mutant embryos. These phenotypes are rescued in embryos that have a single wildtype allele (72/149, 48% of the embryos in D), if embryos inherit maternal message from a wildtype maternal allele (50% of the embryos in E, which are zygotically null but phenotypically normal), or if embryos inherit a single wildtype allele from the male parent (64/64, 100% of the embryos in F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myl7 AB/TU standard conditions Prim-15 heart tube myocardium ISH
s1pr2m93/m93 standard conditions Prim-15 heart tube myocardium ISH
sphk2wcm2/+ standard conditions Prim-15 heart tube myocardium ISH
sphk2wcm2/+ standard conditions Prim-15 heart tube myocardium ISH
sphk2wcm2/+ standard conditions Prim-15 heart tube myocardium ISH
sphk2wcm2/wcm2 standard conditions Prim-15 heart tube myocardium ISH
sphk2wcm2/wcm2 standard conditions Prim-15 heart tube myocardium ISH
sphk2wcm2/wcm2 standard conditions Prim-15 heart tube myocardium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s1pr2m93/m93 standard conditions Prim-15 cell migration to the midline involved in heart development decreased occurrence, abnormal
Prim-15 embryonic heart tube formation decreased occurrence, abnormal
Prim-15 heart edematous, abnormal
Prim-15 heart tube split bilaterally, abnormal
Prim-15 post-vent region epithelium blistered, abnormal
sphk2wcm2/+ standard conditions Prim-15 cell migration to the midline involved in heart development occurrence, normal
sphk2wcm2/+ standard conditions Prim-15 cell migration to the midline involved in heart development occurrence, normal
sphk2wcm2/+ standard conditions Prim-15 cell migration to the midline involved in heart development occurrence, normal
sphk2wcm2/+ standard conditions Prim-15 embryonic heart tube formation occurrence, normal
sphk2wcm2/+ standard conditions Prim-15 embryonic heart tube formation occurrence, normal
sphk2wcm2/+ standard conditions Prim-15 embryonic heart tube formation occurrence, normal
sphk2wcm2/+ standard conditions Prim-15 heart tube morphology, normal
sphk2wcm2/+ standard conditions Prim-15 heart tube morphology, normal
sphk2wcm2/+ standard conditions Prim-15 heart tube morphology, normal
sphk2wcm2/+ standard conditions Prim-15 post-vent region epithelium morphology, normal
sphk2wcm2/+ standard conditions Prim-15 post-vent region epithelium morphology, normal
sphk2wcm2/+ standard conditions Prim-15 post-vent region epithelium morphology, normal
sphk2wcm2/wcm2 standard conditions Prim-15 cell migration to the midline involved in heart development decreased occurrence, abnormal
sphk2wcm2/wcm2 standard conditions Prim-15 cell migration to the midline involved in heart development decreased occurrence, abnormal
sphk2wcm2/wcm2 standard conditions Prim-15 cell migration to the midline involved in heart development occurrence, normal
sphk2wcm2/wcm2 standard conditions Prim-15 embryonic heart tube formation decreased occurrence, abnormal
sphk2wcm2/wcm2 standard conditions Prim-15 embryonic heart tube formation decreased occurrence, abnormal
sphk2wcm2/wcm2 standard conditions Prim-15 embryonic heart tube formation occurrence, normal
sphk2wcm2/wcm2 standard conditions Prim-15 heart edematous, abnormal
sphk2wcm2/wcm2 standard conditions Prim-15 heart edematous, abnormal
sphk2wcm2/wcm2 standard conditions Prim-15 heart tube morphology, normal
sphk2wcm2/wcm2 standard conditions Prim-15 heart tube split bilaterally, abnormal
sphk2wcm2/wcm2 standard conditions Prim-15 heart tube split bilaterally, abnormal
sphk2wcm2/wcm2 standard conditions Prim-15 post-vent region epithelium blistered, abnormal
sphk2wcm2/wcm2 standard conditions Prim-15 post-vent region epithelium blistered, abnormal
sphk2wcm2/wcm2 standard conditions Prim-15 post-vent region epithelium morphology, normal
Acknowledgments:
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